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Jackson Laboratory cx40 egfp
( A ) Experimental design to assess expression of VegfR2 and endomucin in single artery endothelial cells (saECs), post-MI. ( B , C ) Quantification of ( B ) VegfR2 and ( C ) Endomucin, in saECs, post-MI. p<0.0001. Each data point is a TdTomato + single artery cell. N=3 and N=5 hearts were quantified for VegfR2 and Endomucin expression, respectively. ( D ) Confocal images of saECs in Cx40CreER; Rosa26 TdTomato ; <t>Cx40</t> eGFP/+ hearts, post-MI, showing TdTomato + saECs in red and expression of eGFP as a reporter for Cx40 expression in green. Arrowheads point to TdTomato + saECs with low levels of eGFP signal, as compared to the artery tip. ( E , H ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for VegfR2 in green in ( E ) control ( Cx40CreER; Rosa26 TdTomato ) and ( F ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. F , G are insets from E and show TdTomato + saECs express VegfR2 (orange arrowheads). I , J are insets from H and show reduced expression of VegfR2 by TdTomato + saECs (magenta arrowheads). ( K , N ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for Endomucin in green in ( K ) control ( Cx40CreER; Rosa26 TdTomato ) and ( N ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. L , M are insets from K and show TdTomato + saECs express Endomucin (orange arrowheads). O , P are insets from N and show reduced expression of Endomucin by TdTomato + saECs (magenta arrowheads). Scalebars: 50µm. saECs, single artery endothelial cells; P, postnatal day; KO, knockout; cap, capillary; MI, myocardial infarction.
Cx40 Egfp, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cx40 egfp/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
cx40 egfp - by Bioz Stars, 2026-06
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1) Product Images from "Cell-autonomous Wnt activity promotes transient re-programming and cell cycle re-entry of coronary artery endothelial cells"

Article Title: Cell-autonomous Wnt activity promotes transient re-programming and cell cycle re-entry of coronary artery endothelial cells

Journal: bioRxiv

doi: 10.64898/2026.02.23.707374

( A ) Experimental design to assess expression of VegfR2 and endomucin in single artery endothelial cells (saECs), post-MI. ( B , C ) Quantification of ( B ) VegfR2 and ( C ) Endomucin, in saECs, post-MI. p<0.0001. Each data point is a TdTomato + single artery cell. N=3 and N=5 hearts were quantified for VegfR2 and Endomucin expression, respectively. ( D ) Confocal images of saECs in Cx40CreER; Rosa26 TdTomato ; Cx40 eGFP/+ hearts, post-MI, showing TdTomato + saECs in red and expression of eGFP as a reporter for Cx40 expression in green. Arrowheads point to TdTomato + saECs with low levels of eGFP signal, as compared to the artery tip. ( E , H ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for VegfR2 in green in ( E ) control ( Cx40CreER; Rosa26 TdTomato ) and ( F ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. F , G are insets from E and show TdTomato + saECs express VegfR2 (orange arrowheads). I , J are insets from H and show reduced expression of VegfR2 by TdTomato + saECs (magenta arrowheads). ( K , N ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for Endomucin in green in ( K ) control ( Cx40CreER; Rosa26 TdTomato ) and ( N ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. L , M are insets from K and show TdTomato + saECs express Endomucin (orange arrowheads). O , P are insets from N and show reduced expression of Endomucin by TdTomato + saECs (magenta arrowheads). Scalebars: 50µm. saECs, single artery endothelial cells; P, postnatal day; KO, knockout; cap, capillary; MI, myocardial infarction.
Figure Legend Snippet: ( A ) Experimental design to assess expression of VegfR2 and endomucin in single artery endothelial cells (saECs), post-MI. ( B , C ) Quantification of ( B ) VegfR2 and ( C ) Endomucin, in saECs, post-MI. p<0.0001. Each data point is a TdTomato + single artery cell. N=3 and N=5 hearts were quantified for VegfR2 and Endomucin expression, respectively. ( D ) Confocal images of saECs in Cx40CreER; Rosa26 TdTomato ; Cx40 eGFP/+ hearts, post-MI, showing TdTomato + saECs in red and expression of eGFP as a reporter for Cx40 expression in green. Arrowheads point to TdTomato + saECs with low levels of eGFP signal, as compared to the artery tip. ( E , H ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for VegfR2 in green in ( E ) control ( Cx40CreER; Rosa26 TdTomato ) and ( F ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. F , G are insets from E and show TdTomato + saECs express VegfR2 (orange arrowheads). I , J are insets from H and show reduced expression of VegfR2 by TdTomato + saECs (magenta arrowheads). ( K , N ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for Endomucin in green in ( K ) control ( Cx40CreER; Rosa26 TdTomato ) and ( N ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. L , M are insets from K and show TdTomato + saECs express Endomucin (orange arrowheads). O , P are insets from N and show reduced expression of Endomucin by TdTomato + saECs (magenta arrowheads). Scalebars: 50µm. saECs, single artery endothelial cells; P, postnatal day; KO, knockout; cap, capillary; MI, myocardial infarction.

Techniques Used: Expressing, Immunostaining, Control, Knock-Out



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Jackson Laboratory cx40 egfp
( A ) Experimental design to assess expression of VegfR2 and endomucin in single artery endothelial cells (saECs), post-MI. ( B , C ) Quantification of ( B ) VegfR2 and ( C ) Endomucin, in saECs, post-MI. p<0.0001. Each data point is a TdTomato + single artery cell. N=3 and N=5 hearts were quantified for VegfR2 and Endomucin expression, respectively. ( D ) Confocal images of saECs in Cx40CreER; Rosa26 TdTomato ; <t>Cx40</t> eGFP/+ hearts, post-MI, showing TdTomato + saECs in red and expression of eGFP as a reporter for Cx40 expression in green. Arrowheads point to TdTomato + saECs with low levels of eGFP signal, as compared to the artery tip. ( E , H ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for VegfR2 in green in ( E ) control ( Cx40CreER; Rosa26 TdTomato ) and ( F ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. F , G are insets from E and show TdTomato + saECs express VegfR2 (orange arrowheads). I , J are insets from H and show reduced expression of VegfR2 by TdTomato + saECs (magenta arrowheads). ( K , N ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for Endomucin in green in ( K ) control ( Cx40CreER; Rosa26 TdTomato ) and ( N ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. L , M are insets from K and show TdTomato + saECs express Endomucin (orange arrowheads). O , P are insets from N and show reduced expression of Endomucin by TdTomato + saECs (magenta arrowheads). Scalebars: 50µm. saECs, single artery endothelial cells; P, postnatal day; KO, knockout; cap, capillary; MI, myocardial infarction.
Cx40 Egfp, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cx40 egfp/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
cx40 egfp - by Bioz Stars, 2026-06
86/100 stars
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( A ) Experimental design to assess expression of VegfR2 and endomucin in single artery endothelial cells (saECs), post-MI. ( B , C ) Quantification of ( B ) VegfR2 and ( C ) Endomucin, in saECs, post-MI. p<0.0001. Each data point is a TdTomato + single artery cell. N=3 and N=5 hearts were quantified for VegfR2 and Endomucin expression, respectively. ( D ) Confocal images of saECs in Cx40CreER; Rosa26 TdTomato ; Cx40 eGFP/+ hearts, post-MI, showing TdTomato + saECs in red and expression of eGFP as a reporter for Cx40 expression in green. Arrowheads point to TdTomato + saECs with low levels of eGFP signal, as compared to the artery tip. ( E , H ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for VegfR2 in green in ( E ) control ( Cx40CreER; Rosa26 TdTomato ) and ( F ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. F , G are insets from E and show TdTomato + saECs express VegfR2 (orange arrowheads). I , J are insets from H and show reduced expression of VegfR2 by TdTomato + saECs (magenta arrowheads). ( K , N ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for Endomucin in green in ( K ) control ( Cx40CreER; Rosa26 TdTomato ) and ( N ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. L , M are insets from K and show TdTomato + saECs express Endomucin (orange arrowheads). O , P are insets from N and show reduced expression of Endomucin by TdTomato + saECs (magenta arrowheads). Scalebars: 50µm. saECs, single artery endothelial cells; P, postnatal day; KO, knockout; cap, capillary; MI, myocardial infarction.

Journal: bioRxiv

Article Title: Cell-autonomous Wnt activity promotes transient re-programming and cell cycle re-entry of coronary artery endothelial cells

doi: 10.64898/2026.02.23.707374

Figure Lengend Snippet: ( A ) Experimental design to assess expression of VegfR2 and endomucin in single artery endothelial cells (saECs), post-MI. ( B , C ) Quantification of ( B ) VegfR2 and ( C ) Endomucin, in saECs, post-MI. p<0.0001. Each data point is a TdTomato + single artery cell. N=3 and N=5 hearts were quantified for VegfR2 and Endomucin expression, respectively. ( D ) Confocal images of saECs in Cx40CreER; Rosa26 TdTomato ; Cx40 eGFP/+ hearts, post-MI, showing TdTomato + saECs in red and expression of eGFP as a reporter for Cx40 expression in green. Arrowheads point to TdTomato + saECs with low levels of eGFP signal, as compared to the artery tip. ( E , H ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for VegfR2 in green in ( E ) control ( Cx40CreER; Rosa26 TdTomato ) and ( F ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. F , G are insets from E and show TdTomato + saECs express VegfR2 (orange arrowheads). I , J are insets from H and show reduced expression of VegfR2 by TdTomato + saECs (magenta arrowheads). ( K , N ) Confocal images of post-MI watersheds, showing TdTomato + saECs in red and immunostaining for Endomucin in green in ( K ) control ( Cx40CreER; Rosa26 TdTomato ) and ( N ) knockout ( Fzd4 L/L ; Cx40CreER; Rosa26 TdTomato ) MI hearts. L , M are insets from K and show TdTomato + saECs express Endomucin (orange arrowheads). O , P are insets from N and show reduced expression of Endomucin by TdTomato + saECs (magenta arrowheads). Scalebars: 50µm. saECs, single artery endothelial cells; P, postnatal day; KO, knockout; cap, capillary; MI, myocardial infarction.

Article Snippet: Following mouse lines, Cx40CreER , ApjCreER , Cdk1 fl/fl (The Jackson Laboratory, 129S(B6N)-Cdk1 tm1Eddy /J, stock number 028028), Fzd4 fl/fl (The Jackson Laboratory, B6;129-Fzd4 tm2.1Nat /J, stock number 011078), Wls fl/fl (The Jackson Laboratory, Wls tm1.1Whsu /J, stock number 027484), Rosa26 TdTomato Cre reporter line (The Jackson Laboratory, B6.Cg-Gt[ROSA]26Sortm9[CAG-TdTomato]Hze/J, stock number 007909), Cx40 eGFP/+ and Axin2-d2EGFP were used in the experiments.

Techniques: Expressing, Immunostaining, Control, Knock-Out